Open AccessExtraction of Estrogen Hormones from Water Using Carbon Cryogel as Sorbent
Author(s) -
Danijela Prokić,
Marija Vukčević,
Marina Maletić,
Ana Kalijadis,
Biljana Babić,
Ivona Janković-Čaštvan,
Tatjana Đurkić
Publication year - 2021
Language(s) - English
Resource type - Conference proceedings
DOI - 10.24094/ptk.021.34.1.123
Subject(s) - sorbent , solid phase extraction , chromatography , estrone , extraction (chemistry) , chemistry , elution , ethinylestradiol , hormone , adsorption , organic chemistry , biochemistry , medicine , population , environmental health , research methodology
In this study, carbon cryogel was used as solid-phase extraction sorbent for the extraction of estrogenic hormones (estrone, 17β-estradiol, and 17α-ethinylestradiol) from water solution. The solid-phase extraction (SPE) method was optimized by choosing an appropriate mass of the sorbent, volume, and initial pH of estrogenic hormone water solution, as well as by choosing an appropriate organic solvent. The concentration of tested hormones after extraction was measured by liquid chromatography coupled with tandem mass spectrometry. Based on the obtained hormone recoveries, the following optimal conditions of the SPE procedure were chosen: 100 cm3 of hormone water solution at initial pH adjusted to 7, 20 mg of the sorbent, the methanol-dichloromethane mixture was used for hormone elution. Recoveries obtained under the optimal conditions ranged from 77 % for estrone, to 86 % for 17β-estradiol, with relative standard deviation from 7,4 to 18 %.