Open AccessDEVELOPMENT OF REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR SIMULTANEOUS ESTIMATION OF SILDENAFIL CITRATE AND DEPOXETINE HYDROCHLORIDE IN PHARMACEUTICAL FORMULATION
Author(s) -
SHIVANAND YADAV,
Neeraj Sharma
Publication year - 2022
Publication title -
current research in pharmaceutical sciences
Language(s) - English
Resource type - Journals
ISSN - 2250-2688
DOI - 10.24092/crps.2022.120104
Subject(s) - chromatography , sildenafil , high performance liquid chromatography , dosage form , chemistry , chromatography detector , reversed phase chromatography , medicine
Simple, accurate and precise reversed-phase high-performance liquid chromatographic (RP-HPLC) methods for simultaneous estimation of sildenafil citrate (SIL) and depoxetine hydrochloride (DAP) in combined tablet dosage form have been developed and validated. The RP-HPLC method uses a Shimadzu – 1800, Software Version – UV Prob 2.33 with BDS hypersil C18 column and mixture of Buffer pH 4.0 and ACN in the ratio of 40:60 as the mobile phase. The detection was carried out using a UV–Visible Detector: Shimadzu SPD–20AT diode array detector set at 229 nm. Linearity of chromatographic method was found in the concentration range of 50 - 150 ppm for Sildenafil and 30 - 90 ppm for Dapoxetine respectively. % recovery for SIL was found to be 99.3 – 100.5 %, while for DAP it was found to be in range of 99.1 – 100.4 % in HPLC methods respectively. Both methods may be used for routine analysis of the drugs in a pharmaceutical formulation. Results of analysis were validated statistically. KEYWORDS: Linearity, Validation, HPLC, Sildenafil, Depoxetine.