Open AccessAnalysis of the impact of long non-coding RNA gene polymorphisms (ANRIL, MALAT1, HOTAIR) on some clinical and pathologic features in patients with bladder cancer
Author(s) -
A. D. Volkohon,
V. Yu. Harbuzova,
I. Danilishin,
D. Nechyporenko,
Олександр Васильович Атаман
Publication year - 2021
Publication title -
bukovinsʹkij medičnij vìsnik
Language(s) - English
Resource type - Journals
eISSN - 2413-0737
pISSN - 1684-7903
DOI - 10.24061/2413-0737.xxv.2.98.2021.3
Subject(s) - genotyping , genotype , bladder cancer , hotair , single nucleotide polymorphism , polymerase chain reaction , restriction fragment length polymorphism , locus (genetics) , gene , gastroenterology , biology , renal cell carcinoma , medicine , microbiology and biotechnology , oncology , cancer , genetics , long non coding rna , rna
Objective – to analyze the possible association between the SNPs rs4977574 (ANRIL gene), rs3200401 (MALAT1 gene), rs1899663 (HOTAIR gene) and tumor size and some clinical and laboratory testing data in patients with transitional cell carcinoma of the urinary bladder (TCCUB).Material and methods. Whole venous blood of 141 patients with TCCUB was used. The genotyping of rs4977574 and rs3200401 sites was performed by real-time polymerase chain reaction (Real-time PCR). The rs1899663 locus genotyping was done by polymerase chain reaction followed by restriction fragment length polymorphism analysis (PCR-RFLP). Mathematical analysis of the obtained data was performed using the software package SPSS (version 17.0).Results. It was found that individuals with TT-genotype (rs3200401-polymorphism) have a lower blood hemoglobin content ((106.3 ± 23.9) g/l; P = 0.043) and higher blood glucose ((7.1 ± 2.3) mmol/l; P = 0.043) and creatinine ((104.5 ± 33.8) μmol/l; P = 0.022) than patients with CC genotype (respectively: (131.1 ± 21.9) g/l); (5.4 ± 1.5) mmol/l); (83.8 ± 18.5) μmol/l)). TT-homozygotes also have the higher tumor width ((4.2 ± 1.7) cm; P = 0.027) than in CC-homozygotes ((2.9 ± 1.1) cm). No significant association between rs4977574, rs1899663 loci and studied parameters was found.Conclusion. The MALAT1 gene rs3200401 polymorphism is associated with tumor size and blood hemoglobin, glucose, and creatinine levels in patients with TCCUB. No association between rs1899663, rs4977574 loci and clinical and pathological features in patients with TCUUB was detected.