Experimental justification of the method of endoscopic hemostasis

To study the possibility and effectiveness of using succinylated gelatin (SG) for injecting endoscopic hemostasis. Material and methods. 70 albino non-linear rats. 0.5 ml of the solution has been injected into the anterior wall of the stomach with a syringe after laparotomy. A mixture of 0.9 % NaCl solution and epinephrine hydrothartrate (1.8 mg/1 ml) has been used in a ratio of 1:20 for the control group (35 animals). A mixture of a solution of SG (40 mg/1000 ml of injection water) and epinephrine hydrothartrate (1.8 mg/1 ml) in a ratio of 1:20 for the experimental group (35 animals). Right after the injection and in 1, 3, 6, 12 hours, after laparotomy, the stomach wall has been taken for histological examination, during which the thickness of the stomach wall (TSW) has been determined. Results. As soon as the injection has been given, the TSW in animals of both groups hasn`t differed significantly. The TSW has significantly increased in both of the groups, and the parameters of indicators in the experimental group have significantly raised in 1 h after the injection. In 3, 6, and 12 hours, the TSW in both groups has been decreasing. The parameters of indicators in the experimental group of animals have been significantly higher than in the control one all the time during the study. The indicators have barely differed from the initial ones in the control group 12 hours later, whereas in the experimental one they have been significantly higher. Histological studies have shown that the structure of stomach tissues in both groups hasn`t varied. The SG injection has not caused any negative effects on the tissues of the stomach wall. The thickness of the submucosa hasn`t been much modified right after the injection. The thickness (26.95±5.34 microns) has increased in the experimental group 12 hours later than in the control one (15.45±4.38 microns, p<0.01). Most of the vessels of the experimental group have remained compressed 12 hours later, whereas the diameter of the vessels of the control group has been growing. Conclusions. 1. The TSW hasn`t differed right after the injection (a mixture of 0.9 % NaCl and epinephrine solution and a mixture of SG and epinephrine solution) into the stomach wall. 2. 1 h later after the injection, the TSW has increased and its parameters after injection of a mixture with SG have been significantly higher. 3. In 3,6,12 h and later on, the TSW has been gradually reducing and its parameters after injection of a mixture with SG have been significantly higher each time. The thickness of the submucosa and the degree of compression of the vessels have also been considerably higher. 4. No negative changes in the structure of stomach tissues after injection of the mixture with SG have been detected. This allows it to be used for performing endoscopic hemostasis by injection therapy in clinical conditions.