Open AccessHISTOLOGICAL AND IMMUNOHISTOCHEMICAL ANALYSIS OF CONNECTIVE TISSUE CHANGES IN PATIENTS WITH ENTEROCUTANEOUS FISTULA
Author(s) -
Ya. Yu. Voitiv,
О. Yu. Usenko,
О. О. Dyadyk
Publication year - 2020
Publication title -
klìnìčna anatomìâ ta operativna hìrurgìâ
Language(s) - English
Resource type - Journals
eISSN - 1993-5897
pISSN - 1727-0847
DOI - 10.24061/1727-0847.19.1.2020.17
Subject(s) - connective tissue , pathology , immunohistochemistry , enterocutaneous fistula , basement membrane , monoclonal antibody , soft tissue , dense connective tissue , medicine , biology , fistula , antibody , immunology , surgery
We examined 43 patients with enterocutaneous fistula and connective tissue pathology, who were treated in the Shalimov National Institute of Surgery and Transplantology during 2017-2020.With comprehensive study of tissue fragments small and large intestines revealed similar morphological characteristics in groups of phenotypic traits undifferentiated connective tissue dysplasia and with external intestinal fistula. In immunohistochemical study of tissues with monoclonal antibodies to α-SMA in both groups revealed uneven focal expression in smooth muscle cells and fibroblast; with monoclonal antibodies to Collagen IV there is a moderate positive expression in the basement membrane of blood vessels, in smooth muscle cells of the muscular layer of the vascular wall, in areas of connective tissue, which are signs of pathological remodeling of connective tissue.