Open AccessQuinolines and Macrolides Resistance-Associated Mutations in Chlamydia trachomatis in Women Endocervical Samples in the West Region of Cameroon
Author(s) -
B. E. Djoumessi Gomseu,
Rajneesh Dadwal,
Jean-De-Dieu Tamokou,
Rakesh Yadav,
Wiliane Jean Takougoum Marbou,
Jules Roger Kuiaté,
Sunil Sethi
Publication year - 2021
Publication title -
european journal of medical and health sciences
Language(s) - English
Resource type - Journals
ISSN - 2593-8339
DOI - 10.24018/ejmed.2021.3.5.1071
Subject(s) - 23s ribosomal rna , chlamydia trachomatis , quinolone , antibiotics , biology , mutation , microbiology and biotechnology , drug resistance , gene mutation , gene , macrolide antibiotics , mutation rate , antibiotic resistance , virology , genetics , erythromycin , ribosome , rna
Chlamydia trachomatis infection is a public health problem worldwide. Although antibiotic resistance of this strict intracellular bacterium is rare, it is important to monitor the appearance of resistance genes to available efficient antibiotics. This study aimed to screen for mutations in some of these genes in C. trachomatis clinical isolates, which may be associated to resistance to quinolone and macrolide antibiotics. Thirty-five endocervical samples were collected from women aged between 18 and 49 in five district hospitals in the Western Region of Cameroon. The mutations in quinolones (parC and gyrA) and macrolides (L4, L22 and 23S rRNA) resistance domains were detected by PCR followed by sequencing on positive samples to C. trachomatis. The overall mutation rate for the studied genes was 60% in the studied samples. Seven (20%) and twelve (34%) samples presented mutations in the parC and gyrA gene respectively. Mutations in L4 (11.42%) and L22 (60%) were detected in ours samples, while no mutation was found in 23S rRNA gene. Seven clinical samples (20%) presented mutations to both macrolide and quinolone resistance genes. This study revealed a relatively high rate of mutations in the resistance genes to macrolides and quinolones in C. trachomatis in the West Cameroon. This rate of mutation calls for the competent authorities for better surveillance of C. trachomatis infection in West Cameroon to avoid a sudden increase in resistance to antibiotics in the years to come.