Open AccessAnti-Hyperglycaemic and Anti-Oxidative Effect of Merremia Tridentata (Linn) Hallier F Ethanol Leaf Extract
Author(s) -
Olubodun A. Adebiyi,
D. A. Ameh,
Elewechi Onyike,
Dorcas Bolanle James
Publication year - 2022
Publication title -
european journal of advanced chemistry research
Language(s) - English
Resource type - Journals
ISSN - 2684-4478
DOI - 10.24018/ejchem.2022.3.1.87
Subject(s) - tbars , thiobarbituric acid , superoxide dismutase , chemistry , antioxidant , catalase , glutathione , kidney , endocrinology , medicine , zoology , lipid peroxidation , biology , biochemistry , enzyme
The anti-hyperglycaemic and anti-oxidative effect of Merremia tridentata (Linn) Hallier F ethanol leaf extract (MTELE) was evaluated in this study. Diabetes was induced on male albino wistar rats by a single intra-peritoneal injection of 65mg/kg body weight dose of streptozotocin. The rats were divided into seven groups and treated accordingly with varying doses (50 mg/kg, 75 mg/kg and 100 mg/kg body weight) of MTELE for seven weeks. Body weight and plasma glucose levels were monitored while antioxidant parameters, catalase (CAT), superoxide dismutase (SOD) activities, reduced glutathione (GSH) concentration and levels of thiobarbituric acid reacting substances (TBARS) were determined in the liver and kidney tissues. There was a significant (p<0.05) reduction in the weight of the diabetic control group which was significantly (p<0.05) ameliorated by the treatment. Plasma glucose levels were significantly (p<0.05) reduced by 50.6%, 62.1% and 72.68% in diabetic rats treated with 50 mg/kg, 75 mg/kg and 100mg/kg body weight dose of MTELE respectively. There was a significant (p<0.05) improvement in all the antioxidant parameters investigated in this study in both the liver and kidney tissues at all levels of treatment (increase in the activities of CAT and SOD, increase in tissue content of GSH and drastic reduction of TBARS level). The extract at 100mg/kg body weight dose on normal rats showed a significant (p<0.05) improvement in the status of SOD in the kidney, GSH and TBARS in the liver when compared with the normal control group. MTELE demonstrated a good anti-hyperglycaemia and antioxidant potential and should be further investigated for development of lead compound capable of preventing and treating oxidative stress related complications in diabetics.