Open AccessFID-Gas Chromatography of West-Algerian Cucurbita pepo (or Pumpkin) Seed Oil Extract
Author(s) -
Abdelkrim Berroukche,
Ammam Abdelkader,
Mohamed Terras,
Mohamed Amine Souidi,
M. Chibani,
Hafsa Habri,
Aicha Amara,
Hafsa Dellaoui,
Farouk Boudou
Publication year - 2021
Publication title -
european journal of advanced chemistry research
Language(s) - English
Resource type - Journals
ISSN - 2684-4478
DOI - 10.24018/ejchem.2021.2.3.69
Subject(s) - cucurbita pepo , campesterol , pumpkin seed , phytosterol , gas chromatography , chemistry , stigmasterol , cucurbita , polyunsaturated fatty acid , fatty acid , food science , sterol , chromatography , botany , biology , cholesterol , organic chemistry , biochemistry
Fatty acid and phytosterol vegetable oils were extracted from seeds of Cucurbita pepo (or pumpkin) into (v/v) hexane. The extract obtained was characterized by the contents of sterols, unsaturated and saturated fatty acids. The content of the bioactive compounds was determined by gas-chromatography FID method. Pumpkin seed oil extracts showed higher content of poly-unsaturated fatty acids (49.10%) than that from saturated fatty acids (28%). Sterols exhibited different molecules dominated by β-sistostérol (47%), Stigmastérol (23.6%) and campesterol (21.5%). Several peaks were present on the FID GC chromatogram of two extracts (fatty acids and sterols respectively). GC spectra confirmed the presence of the predominant bioactive compounds (PUFA, β-sistostérol , Stigmastérol and campesterol). The composition of pumpkin seed oil extract was characterized by FID-GC spectra with maximum at 280 nm.