Open AccessComparative Karyomorphological Analysis of In vivo and In vitro Grown Plants of Plumbago zeylanica: An Important Medicinal Plant
Author(s) -
Sweety Majumder,
Animesh Biswas,
Md. Mahbubur Rahman
Publication year - 2022
Publication title -
european journal of biology and biotechnology
Language(s) - English
Resource type - Journals
ISSN - 2684-5199
DOI - 10.24018/ejbio.2022.3.2.333
Subject(s) - in vivo , biology , ploidy , in vitro , botany , karyotype , somatic cell , chromosome number , chromosome , genetics , gene
The present report describes the comparative cytological analysis of in vivo and in vitro grown plant species of Plumbago zeylanica. Somatic chromosome number of in vivo and micro-propagated plants was confirmed to be 2n=28. Individual chromosome length of mother plant and micro-propagated plant was ranged from 2.26 to 5.54 µm and 2.26 to 5.46 µm, respectively. The total length of the haploid complement of in vivo and in vitro grown plants was 49.35 and 52.98 µm. The total form percent (TF%) of mother and in vitro grown plants was 45.11% and 45.58%, respectively and according to Stebbins classification (1971) both plants karyotype was fell into 2B symmetric type. The centromeric formula was for in vivo and in vitro grown plants were 3sm + 11m.