Open AccessDetecting and Analyzing Hydrolytic Enzymes of Industrial Significance in two Streptomyces Strains Isolated from the Soil
Author(s) -
Olaitan Akintunde
Publication year - 2021
Publication title -
european journal of biology and biotechnology
Language(s) - English
Resource type - Journals
ISSN - 2684-5199
DOI - 10.24018/ejbio.2021.2.3.214
Subject(s) - lipase , cellulase , carboxymethyl cellulose , hydrolysis , amylase , streptomyces , starch , chemistry , biochemistry , casein , cellulose , enzyme , strain (injury) , food science , microbiology and biotechnology , biology , bacteria , organic chemistry , anatomy , genetics , sodium
Two Streptomyces strain were isolated from a soil sample in Louisiana. They were identified via 16S rRNA sequencing and phylogeny. To detect the presence of hydrolytic enzymes, starch, carboxymethyl-cellulose (CMC), lipase reagent, and Milk (casein) were used as substrate to detect the production of amylase, cellulose, lipase and casease respectively. Both strains showed the ability to hydrolyze starch, and cellulose, while only strain SWHR10 displayed lipase activity. In addition, strain SWHR10 showed better amylase and cellulase activity. Hemolysis, gelatinase and catalase tests were also conducted. This study further validates that Streptomyces remain a powerhouse of hydrolytic enzymes with industrial and economic importance.