Open AccessLeucocytic DNA Damage of Racing and Retired Horses
Author(s) -
Yim Tong Savio Szeto
Publication year - 2021
Publication title -
european journal of biology and biotechnology
Language(s) - English
Resource type - Journals
ISSN - 2684-5199
DOI - 10.24018/ejbio.2021.2.3.204
Subject(s) - dna damage , comet assay , horse , medicine , veterinary medicine , biology , dna , genetics , paleontology
Comet assay was performed on five racing horses (age, mean ± SD: 4.4 ± 0.5 years old) and five retired horses (10.2 ± 4.1 years old). DNA damage of whole blood samples were tested with or without UV irradiation. Three specimens were taken from each horse and one month apart for each specimen to test for reproducibility. Result showed that racing horses had statistically significant lower baseline and UV induced DNA damage in leucocytes than retired horses. Positive associations were also seen between age and baseline DNA damage or UV induced DNA damage from Spearman correlation analysis.