Open AccessAktivitas Antibakteri Ekstrak Karang Lunak Sarcophyton sp. yang Difragmentasi dan Tidak Difragmentasi dari Perairan Pulau Pramuka, Kepulauan Seribu, Jakarta
Author(s) -
Mujizat Kawaroe,
Dedi Soedarma,
Hefni Effendi,
Tati Nurhayati,
Safrina Dyah Hardiningtyas
Publication year - 2019
Publication title -
biota
Language(s) - English
Resource type - Journals
eISSN - 2527-323X
pISSN - 2527-3221
DOI - 10.24002/biota.v15i3.2588
Subject(s) - artemia salina , ethyl acetate , brine shrimp , traditional medicine , coral , antibacterial activity , chemistry , minimum inhibitory concentration , food science , biology , bacteria , chromatography , antibiotics , biochemistry , toxicity , medicine , organic chemistry , ecology , genetics
Fragmented and non-fragmented soft corals showed antibacterial activities. Soft corals were gradually extracted using methanol, ethyl acetate, and hexane. Crude extract of the samples was tested its antibacterial activity, Minimun Inhibitory Concentration, toxicity (Brine Shrimp Lethality Test method), and phytochemicals. Overall, the antibacterial activity of crude extract of non-fragmented soft coral Sarcophyton sp. was higher than the crude extract of fragmented soft coral Sarcophyton sp. Crude ethyl acetate extract showed higher antibacterial activities. The ethyl acetate crude extract of non-fragmented soft coral Sarcophyton sp. is able to inhibit all tested bacteria is E. coli, S. aureus, P. aeruginosa and B.cereus, while the ethyl acetate crude extract of fragmented Sarcophyton sp. is unable to inhibit bacteria P. aeruginosa. Minimum inhibitory concentration extracts of non-fragmented Sarcophyton sp. in range 240−480 μg/disc. The 24-h LC50 extracts of fragmented and non-fragmented Sarcophyton sp. for Artemia salina were 149.50 ppm and 45.15 ppm, respectively. Bioactive compounds of fragmented and non-fragmented Sarcophyton sp. extract are steroid, flavonoid and alkaloid.