Open AccessProduksi Fitoaleksin pada Tusam (Pinus merkusii Jungh. et de Vriese) sebagai Respon Infeksi Fungi Mikorisa
Author(s) -
S. Widyaningsih,
Siti Muslimah Widyastuti,
Sumardi Sumardi
Publication year - 2019
Publication title -
biota
Language(s) - English
Resource type - Journals
eISSN - 2527-323X
pISSN - 2527-3221
DOI - 10.24002/biota.v11i2.2621
Subject(s) - rhizoctonia solani , fusarium solani , inoculation , biology , horticulture , rhizoctonia , botany , root rot , fusarium , antifungal , mycorrhizal fungi , phytoalexin , microbiology and biotechnology , resveratrol , biochemistry
The experiment aimed to detect (1) mycorrhizal infection induced phytoalexins production of the root of tusam and (2) its activity in suppressing a dumping off fungi, Fusarium sp and Rhizoctonia solani. Production of phytoalexins was detected from the extracts of mycorrhizal root of old trees and 4, 6 and 8 week-old seedlings in alcohol using a UV-spectrophotometer. The antifungal activity of phytoalexins was studied by inoculating the pathogenic fungi into mycorrhizal seedlings of tusam. The results showed that the spectra of phytoalexins of extracted mycorrhizal and non-mycorrhizal roots ranged from 203.2 to 204.6 nm. Higher antifungal concentrations of antifungal compound was obtained from the root extracts of old trees and 4 week-old seedlings, indicated by the higher spectrophotometric absorbance (0.315 and 0.324 g-1 root ml-1 ethanol), compared to that extracted from non-mycorrhizal root of the same plant origin. Mycorrhizal roots significantly suppressed the development of Fusarium sp. and Rhizoctonia solani.