Open AccessUniversally primed polymerase chain reaction analysis of Fusarium avenaceum isolated from wheat and barley in Finland
Author(s) -
Tapani Yli-Mattila,
Н. В. Мироненко,
Irina A. Alekhina,
Asko Hannukkala,
С. А. Булат
Publication year - 1997
Publication title -
agricultural and food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.347
H-Index - 35
eISSN - 1795-1895
pISSN - 1459-6067
DOI - 10.23986/afsci.72777
Subject(s) - biology , fusarium , phylogenetic tree , polymerase chain reaction , fungus , host (biology) , pathogenicity , pathogen , poaceae , botany , veterinary medicine , genetics , microbiology and biotechnology , gene , medicine
Twenty-two Fusarium avenaceum isolates from Finnish wheat and barley were analysed using the chain reaction with universal primers (UP-PCR). Each isolate could be distinguished from others by UP-PCR products on polyacrylamide gels. The isolates tested were clustered into two main groups and further into several subgroups by UP-PCR profiles and phylogenetic analyses. The phylogenetic relationships of these groups are discussed. No clear correlation was found between the groups and host plant preference or the geographic origin of F. avenaceum isolates. Pathogenicity tests showed differences between F. avenaceum isolates, but two isolates, one from wheat and the other from barley, were the most aggressive in wheat and barley. This fungus, usually known as a weak pathogen of cereals and other crops, has thus probably not evolved in respect to its ability to damage wheat or barley.