Open AccessMapping antigenic epitopes of potato virus Y with antibodies affinity-purified by using overlapping synthetic peptides
Author(s) -
Maija VihinenRanta,
Reijo Sironen,
Matti Vuento
Publication year - 1994
Publication title -
agricultural and food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.347
H-Index - 35
eISSN - 1795-1895
pISSN - 1459-6067
DOI - 10.23986/afsci.72696
Subject(s) - epitope , polyclonal antibodies , potato virus y , antiserum , antigen , antibody , virology , biology , peptide sequence , microbiology and biotechnology , chemistry , virus , biochemistry , plant virus , genetics , gene
Synthetic, overlapping peptides representing the entire amino acid sequence of potato virus Y (PVY) coat protein were used to affinity-purify antibodies from polyclonal antisera to PVY. In testing the binding of the purified antibodies to PVY particles, antigenic epitopes were identified. The N-terminal and C-terminal regions of the PVY coat protein were found to contain most of the antigenic epitopes. The results will facilitate the development of detection methods for PVY based on synthetic peptides.