The Application of Ectomycoriza In Ex-Limestone Mining Growth Media to Assist the Growth of Mangium (Acacia mangium)

Ex-limestone mining quarry has poor soil fertility, both biological, chemical, and physical condition due to the mining process. Marginal land conditions such as ex-limestone mining quarry require high survival seedlings. Application of ectomycorrhizal fungi is an alternative that can be done to improve the success of restoration. Acacia mangium is one of the plants that could be colonized by many types of mycorrhizal such as ectomycorrhiza. This research aimed to determine the growth of A. mangium seedlings in ex-limestone mining growth media and the effect of ectomycorrhiza application on the growth of A. mangium. The research used a completely randomized design with six treatments and five replications. Treatment consisted of 100% soil with mycorrhiza, 100% soil without mycorrhiza, 50% soil + 50% limestone tailings + mycorrhiza, 50% soil + 50% limestones tailings without mycorrhiza, 100% tailing with mycorrhiza and 100% tailing without mycorrhiza. The seedlings used in the study germinated from seeds taken from Lampung Timur Regency. The seeds germinated in sand for one month, then used as the research material. The measurement taken were  height, diameter, number of leaves, leaf area, measured were root length, canopy dry weight, and total dry weight. The colonization of ectomycorrhiza is measured by counting the percentage of colonization. The symbiosis with rhizobium measured by number of root nodules. Data were tested for variance analysis, followed by Least Significance Different Test at significant level of 1% and 5%. The results showed that the percentage of A. mangium life was high, reaching 100%. All growth parameters showed that plant colonized by mycorrhiza has remarkably better values compared to non-mycorrhiza plants.Keywords: limestone, ectomycoriza, mangium, phytoremediation