Open AccessSELEKSI PSEUDOMONAD FLUORESEN SECARA LANGSUNG DI LAPANGAN UNTUK PENGENDALIAN PENYAKIT LINCAT PADA TEMBAKAU
Author(s) -
Triwidodo Arwiyanto,
Fatma Yuniarsih,
Toekidjo Martoredjo,
dan Gembong Dalmadiyo.
Publication year - 2007
Publication title -
jurnal hama dan penyakit tumbuhan tropika
Language(s) - English
Resource type - Journals
eISSN - 2461-0399
pISSN - 1411-7525
DOI - 10.23960/j.hptt.1762-68
Subject(s) - biology , veterinary medicine , ralstonia solanacearum , horticulture , meloidogyne incognita , pseudomonas , bacterial disease , bacteria , microbiology and biotechnology , pathogen , nematode , medicine , ecology , genetics
Field Screening of Pseudomonad Fluorescent for Controlling Tobacco Lincat Disease. Lincat disease is one of many factors which decreases tobacco production in Temanggung. The disease was caused by a combination between Ralstonia solanacearum and Meloidogyne incognita. The disease is difficult to control by any available control measures. The objective of this research was to find fluorescent pseudomonads which could suppress the lincat disease. The bacteria were isolated from local plants and tested in the field directly without any prior tests in the laboratory. The results indicated that dipping root system of tobacco for 20 minutes in the suspension of fluorescent pseudomonad suppressed lincat disease in the field. The results showed that there were variation in disease index between treatments. Plot treated with isolate pf22, pf23, pf30, pf42, pf51, and pf 83 showed the disease index of 16,67%; 13,34%; 20%; 20%; 16,67%; and 6,67%, respectively. However, there was an inconsistency in the capability of the isolates in the disease suppression. At the second experiment, the plants treated with pf83 isolate showed the disease index of 36,6% with the control plot of 63,3%. Laboratory tests indicated that there were 44 isolates which could inhibit pathogen growth in vitro. The fluorescent pseudomonads used in this experiments were proved not to belong to plant pathogenic bacteria.