Open AccessMolecular markers of cardiac fibrosis after myocardial infarction
Author(s) -
O. L. Barbarash,
Anton G. Kutikhin,
Tamara Pecherina,
R. S. Tarasov,
В. В. Кашталап,
Н. В. Федорова,
Л. А. Богданов,
O. N. Hryachkova,
D. Yu. Sedykh
Publication year - 2022
Publication title -
fundamentalʹnaâ i kliničeskaâ medicina
Language(s) - English
Resource type - Journals
eISSN - 2542-0941
pISSN - 2500-0764
DOI - 10.23946/2500-0764-2022-7-1-17-30
Subject(s) - ctgf , fibrosis , myofibroblast , myocardial infarction , interventricular septum , medicine , extracellular matrix , cardiac fibrosis , myocardial fibrosis , pathology , infarction , cardiology , biology , growth factor , receptor , ventricle , microbiology and biotechnology
Aim. To perform a screening for molecular markers of cardiac fibrosis upon myocardial infarction. Materials and Methods. We carried out echocardiography-guided endomyocardial biopsy of affected and intact interventricular septum segments of 7 patients with anterior myocardial infarction. Fibrotic and adjacent intact cardiac tissue was dissected into 2 equal segments and: 1) homogenized with the further RNA extraction, reverse transcription, and quantitative polymerase chain reaction; 2) fixed in formalin and embedded into paraffin with the further van Gieson staining for the histological verification of cardiac fibrosis. Results. We found that the expression of ACTA2, VIM, CTGF, COL1A1, TGFB1, TGFBR1, AGTR1, CCL2 and TNF genes in fibrotic cardiac tissue was ≥ 3-fold higher as compared with the adjacent intact myocardium reflective of active extracellular matrix production by fibroblast-derived myofibroblasts. Conclusion. We have for the first time shown AGTR1, CCL2, and TNF genes as candidates for post-infarction cardiac fibrosis in addition to ACTA2, VIM, CTGF, COL1A1, TGFB1 , and TGFBR1 genes.