Open AccessEffects of selective vegetative denervation on the urinary bladder of the adult rat
Author(s) -
José Pascual,
Ricardo Insausti,
L.M. Gonzalo
Publication year - 2009
Publication title -
anales del sistema sanitario de navarra
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.175
H-Index - 23
eISSN - 2340-3527
pISSN - 1137-6627
DOI - 10.23938/assn.0595
Subject(s) - wheat germ agglutinin , analysis of variance , tyrosine hydroxylase , urinary bladder , aché , denervation , medicine , sympathetic nervous system , urinary system , cervical ganglia , anatomy , endocrinology , acetylcholinesterase , chemistry , dopamine , lectin , enzyme , biochemistry , blood pressure , immunology
The present work is a study of the plastic capacity of the peripheral nervous system subjected to different aggressions in the case of 28 rats of the Wistar breed. They were divided into four groups: 1) sympathetic deafness (n=9); 2) parasympathetic deafness (n=9); 3) evaluation of regeneration (n=9); 4) control group (n=5). An image analyser was used to study the acetylcholinesterase (AChE) and Tyrosine Hydroxylase (TH) positive ganglionic neurones of the pelvic ganglion (GP) as well as the dorsal ganglions (GRD) compared with the control group. With group 3 a study was also made of the possible plasticity of the transacted axons using a wheat germ agglutinin conjugated-horseradish peroxidase (WGA-HRP) neurotracer. The statistical study was carried out by means of the analysis of variance (ANOVA), Fisher test and Scheffe method, with a p<0.05 taken as significant. The results show the predominant role of the pelvic nerve in the modulation of the plastic changes produced at the ganglionic level, with a lesser influence of the hypogastric nerve. Further studies are needed in order to define the specific role of each of these in the act of miction.