Open AccessCONTROL REGION-mtDNA HETEROGENEITY OF KALIMANTAN FALSE GHARIAL (Tomistoma schlegelii) POPULATION: A PRELIMINARY STUDY
Author(s) -
Hellen Kurniati
Publication year - 2003
Publication title -
berkala penelitian hayati (journal of biological researchers)/berkala penelitian hayati
Language(s) - English
Resource type - Journals
eISSN - 2337-389X
pISSN - 0852-6834
DOI - 10.23869/bphjbr.9.1.20034
Subject(s) - mtdna control region , mitochondrial dna , population , phylogenetic tree , haplotype , biology , geography , ecology , demography , genotype , genetics , sociology , gene
The preliminary genetic study on Kalimantan false gharial from the wild was reported. Eleven tail scutes were collected from eleven individuals that originally consisting of two individuals from Kapuas River, one individual from Sentarum Lake, Jelai River, Mapam River, Perian Lake, and Lamandau River, two individuals from Barito River and three individuals from Mahakam River. PCR amplifying and sequencing 451 nucleotides in average that can be aligned at the same length of control region mitochondrial DNA. Among 11 individuals found eight polymorphic sites that consisted four haplotypes (A, B, C, and D) respectively, which is haplotype A is dominant. Based on phylogenetic tree that constructed by Tamura-Nei parameter, false gharial population in Kalimantan can be divided into two population groups; there were Central-Eastern Kalimantan population group and Western Kalimantan population group. Based on the hypothesis of landmasses separating between central-eastern Kalimantan and western Kalimantan that known as Schwaner Mountains, the genetic distance D = 1.53 percent was expected to be equal to 20 million years.