Open AccessOPTIMASI ANALISIS AMILASE DAN GLUKANASE YANG DIEKSTRAK DARI MISELIUM Pleurotus ostreatus DENGAN ASAM 3,5 DINITROSALISILAT
Author(s) -
Maman Rahmansyah,
I Made Sudiana
Publication year - 2003
Publication title -
berkala penelitian hayati (journal of biological researchers)/berkala penelitian hayati
Language(s) - English
Resource type - Journals
eISSN - 2337-389X
pISSN - 0852-6834
DOI - 10.23869/bphjbr.9.1.20032
Subject(s) - chemistry , amylase , pleurotus ostreatus , food science , reducing sugar , incubation , starch , cellulase , cellulose , substrate (aquarium) , sugar , incubation period , pleurotus , sodium sulfite , enzyme , biochemistry , sodium , mushroom , biology , organic chemistry , ecology
Enzymatic activities of amylase (1.4-a-D-glucan glucano hydrolase) and glucanase (1.4-b-glucanase) that extracted from Pleurotus ostreatus mycelium determined through the reducing sugar accumulation. The enzymes assayed in amorphous cellulose (carboxymethylcellulose = CMC) and starch substrate, respectively. Complexity reaction of the sugar to DNS (3.5-dinitrosalicylic acid) become sensitive caused of adding up oxidative agent of sodium sulfite and phenol. Variation in substrate concentration, combine with incubation period and its temperature was able to promote optimum activities of amylase enzymes. The best possible activity of amylase was turn out on 25° C temperature incubation along 20 minutes in 5 percent starch substrate assessment. Incubation period more then 60 minutes on amylase and cellulase caused those activities leaved to 40 and 70 percent down, correspondingly. Modified DNS fastened on reducing sugar twice effectively compared to unmodified one.