Open AccessInhibitory activity of fermentation filtrate of red passion fruit pulp (Passiflora edulis sims.) against Escherichia coli extended-spectrum beta-lactamase (ESBL) and methicillin resistant Staphylococcus aureus (MRSA)
Author(s) -
Iif Hanifa Nurrosyidah,
Ni Made Mertaniasih,
Isnaeni Isnaeni
Publication year - 2020
Publication title -
berkala penelitian hayati (journal of biological researchers)/berkala penelitian hayati
Language(s) - English
Resource type - Journals
eISSN - 2337-389X
pISSN - 0852-6834
DOI - 10.23869/bphjbr.26.1.20204
Subject(s) - passion fruit , passiflora , staphylococcus aureus , minimum inhibitory concentration , microbiology and biotechnology , chemistry , pulp (tooth) , food science , antibacterial activity , minimum bactericidal concentration , escherichia coli , fermentation , bacteria , biology , horticulture , antimicrobial , biochemistry , medicine , dentistry , genetics , gene
This study aims to determine the minimum inhibitory concentration (MIC) and bactericidal concentration (MBC) of the fermentation filtrate (FF) on red passion fruit pulp (Passiflora edulis Sims.) against Escherichia coli Extended Spectrum Beta Lactamase (ESBL) and Methicillin Resistant Staphylococcus aureus (MRSA). The method used was the fermentation of red passion fruit pulp for 24 h using De Man Rogosa Sharpe Broth (MRS-broth) media, and the result showed that the FF of red passion fruit pulp made a dilution series concentration of 50 percent, 25 percent, 12.5 percent, 6.25 percent. Considering this result, the MIC FF of red passion fruit cells against ESBL and MRSA has a 25 percent dilution concentration, while MBC has a 50 percent dilution concentration. This shows that red passion fruit has the potential to be developed as antibacterial material, especially to fight bacteria that are already resistant.