Open AccessCHITINOLYTIC BACTERY ACTIVITY ISOLATED FROM THE MUD FIELDS
Author(s) -
Nuniek Herdyastuti,
Sari Edi Cahyaningrum,
Tri Joko Raharjo,
Mudasir Mudasir,
Sabirin Mastjeh
Publication year - 2010
Publication title -
berkala penelitian hayati (journal of biological researchers)/berkala penelitian hayati
Language(s) - English
Resource type - Journals
eISSN - 2337-389X
pISSN - 0852-6834
DOI - 10.23869/bphjbr.15.2.20102
Subject(s) - chitinase , chitin , glucosamine , microbiology and biotechnology , chemistry , microorganism , biology , food science , bacteria , biochemistry , chitosan , enzyme , genetics
A chitinolytic microorganism had been isolated from mud fields in Ketintang Surabaya area by using a minimal media containing 0.4% colloidal chitin. Activity assay is based on released N-acetyl-glucosamine which reacted with 3,5 dinitrosalicyclic acid. Mud fields produce 63 bacterial isolates with chitinase activity (TNH1 – TNH63 isolates). The highest activity was shown by TNH11 isolate with specific activity 1.27 U/mg. TNH11 isolates was Gram negative, rod-cocoid cell, has a colony of yellow, round shape, convex elevation, flat margin and the colony diameter 3–5 mm.