Open AccessKAPANG ENDOFIT DARI TAMAN NASIONAL GUNUNG HALIMUN SEBAGAI PENGHAMBAT PERTUMBUHAN MIKROBA PATOGEN Salmonella thypi DAN Candida albicans
Author(s) -
Ruth Melliawati,
Puspita Suci Wulandari
Publication year - 2008
Publication title -
berkala penelitian hayati (journal of biological researchers)/berkala penelitian hayati
Language(s) - English
Resource type - Journals
eISSN - 2337-389X
pISSN - 0852-6834
DOI - 10.23869/bphjbr.13.2.20082
Subject(s) - candida albicans , hypha , antimicrobial , microbiology and biotechnology , biology , agar , plant use of endophytic fungi in defense , chloramphenicol , salmonella , corpus albicans , antibiotics , botany , bacteria , genetics
Endophytic fungi live inside plants without harming the host. The purpose of this research was to screen endophytic fungi which could inhibit Salmonella thypi and Candida albicans, as well as characterizing of endophytic fungi, and antimicrobial compound produced by endophytic fungi. Some methods were used on this research. Diffusion agar plate methode was used to screen endophytic fungi which could produce antimicrobial compound against Salmonella thypi and Candida albicans. Standard plate count was used to measure fungi growth. Antimicrobial compound produced by endophytic fungi was analized by TLC and HPLC technique, compared with standard antibiotic, chloramphenicol and nystatin. The result showed 5 kinds of endophytic fungi produced antimicrobial compounds against Salmonella thypi. The largest zone of inhibition was 115 mm2 shown by Hl.25F.112. Two kinds of endophytic fungi were able to inhibit Candida albicans with the largest inhibiting zone was 164 mm2 shown by Hl.108F.481. The morphology of Hl.108F.481 indicated that this fungus had vertical hypha with sporangium at the end of the hypha and Hl.25F.112 had partioned hypha and oval-shape ascus. TLC and HPLC analysis showed that water extract of Hl.25F.112 (1s), chloroform extract of Hl.25F.112 (1c) and chloroform extract of Hl.108F.481 (2c) contained anti-microbial compound with retention time (RT) closed to chloramphenicol and water phase of Hl.25F.112 (1s) closed to nystatin.