Open Access8-HIDROKSI-DEOKSIGUANOSIN SEBAGAI SALAH SATU INDIKATOR INFERTILITAS PRIA
Author(s) -
Sudjarwo Sudjarwo
Publication year - 2004
Publication title -
berkala penelitian hayati (journal of biological researchers)/berkala penelitian hayati
Language(s) - English
Resource type - Journals
eISSN - 2337-389X
pISSN - 0852-6834
DOI - 10.23869/bphjbr.10.1.20048
Subject(s) - sperm , oligospermia , sperm motility , chemistry , dna , guanine , microbiology and biotechnology , semen , andrology , motility , mitochondrial dna , dna damage , deoxyguanosine , reactive oxygen species , dna oxidation , male infertility , biochemistry , nucleotide , biology , genetics , infertility , medicine , gene , pregnancy
DNA in human’s sperm is located in nucleus (nDNA) and mitochondria (mtDNA). DNA oxidation is indicated from high concentration of 8-OH-dG. It is very important to identify factors that can cause genetic dysfunction risk responsible for genetic changes. 8-OH-dG compound are a promutagenic of DNA dysfunction of deoxyguanosine oxidation by oxygen radical. ROS is an oxidation, which in high concentration can oxidize DNA. Guanine is a nucleotide, which prone to oxidation, with 8-OH-dG as the end product. Detection of 8-OH-dG can be done by HPLC method. These results indicated that there were significant differences (p less than 0.01) in detection of 8-OH-dG in sperm, between normospermia with asthenospermia and normospermia with oligospermia. Correlation (r) of 8-OH-dG with motile spermatozoa is – 0.7111. Immotile spermatozoa caused by oxidation or DNA damage are shown by high concentration of 8-OH-dG. In normal physical condition, sperm produces oxidant. High oxidant is toxic to sperm, which influence their motility. Sperms motility is one of factors causing male infertility.