Open AccessAntimicrobial activity of Micrococcus luteus Cartenoid pigment
Author(s) -
Huda Zuheir Majeed
Publication year - 2017
Publication title -
maǧallaẗ ʻulūm al-mustanṣiriyyaẗ/mustansiriyah journal of science
Language(s) - English
Resource type - Journals
eISSN - 2521-3520
pISSN - 1814-635X
DOI - 10.23851/mjs.2017.1.1.9
Subject(s) - micrococcus luteus , pigment , antimicrobial , antibacterial activity , food science , micrococcus , biology , biofilm , microbiology and biotechnology , pseudomonas aeruginosa , extraction (chemistry) , bacteria , chemistry , chromatography , staphylococcus aureus , organic chemistry , genetics
Cartenoids are group of pigments ,with enormous types different structurally and functionally, have colors range from red to yellow found in a wide variety of plants, fungi, algae and bacteria. The animals took from food because they cannot made it, on contrary, the plants and microbes produce them due to subjection to environment. The aim of the study is to isolate and characterize the cartenoid pigment from Micrococcus luteus. The pigment extraction was done by acetone, then was characterized with Ultra Violet –Visible spectroscopy (UV–Vis) and Fourier Transform Infrared (FTIR) spectroscopy. Then, it was tested for antibacterial activity against five different bacterial isolates and antifungal activity tests against six different fungal isolates by well diffusion method. The results found that, the extracted pigment having antibacterial activity and antifungal activity and having the ability to absorb UVA rays within the range of 300-500 nm. There were no significant difference in antimicrobial effect of pigment , even when the extraction and isolation were done by two culture medium (Nutrient Broth and Luria Bertani Broth). There were considerable inhibition percentages of adhesion after subjection to Cartenoid pigment ranged between (5.71, 23.84) % for Klebsiella spp. and Pseudomonas aeruginosa respectively and all the 11 isolate changed from Biofilm producer to non-producer. The isolated compound can be used against different bacterial and fungal infections. So they had a great future in medicine, cosmetics and as a sun protecting agent.