Open AccessVirulence modulation of Streptococcus mutans biofilms by metal ions released from orthodontic appliances
Author(s) -
Alinne Ulbrich Mores Rymovicz,
Maiara Medeiros Ronsani,
Ana Maria Trindade Grégio,
Odilon Guariza Filho,
Orlando Motohiro Tanaka,
Edvaldo Antônio Ribeiro Rosa
Publication year - 2013
Publication title -
the angle orthodontist
Language(s) - English
Resource type - Journals
eISSN - 1945-7103
pISSN - 0003-3219
DOI - 10.2319/112712-904.1
Subject(s) - streptococcus mutans , biofilm , virulence , saliva , microbiology and biotechnology , chemistry , metal , metal ions in aqueous solution , sugar , dentistry , food science , biology , medicine , bacteria , biochemistry , genetics , organic chemistry , gene
Objectives: To evaluate the impact of metal ions commonly shed from orthodontic appliances on the virulence of Streptococcus mutans ATCC®25175™ biofilms. Materials and Methods: Biofilms were grown in the presence of Ni2+, Fe3+, Cr3+, Co2+, and a metal ion pool at concentrations similar to those released in saliva of orthodontic patients for 72 hours. Once mature, biofilms were treated for up to 12 hours with 5% glucose. Results: Ions interfered with the growth of S mutans by reducing its biomass (Ni2+, Fe3+, Cr3+), raising its rates of sugar metabolism (Ni2+, Fe3+, Cr3+), and raising its secretion of lactate (Ni2+, Fe3+, Cr3+, pool). Conclusion: The laboratory data presented here point to the possibility of virulence increase of S mutans by metal ions commonly released during orthodontic therapy.
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