Apoptosis of Growth Plate Chondrocytes Occurs through a Mitochondrial Pathway | Zendy

Cristina C. Teixeira | Zendy; Aida P. Padron Costas | Zendy; Yeleemelivsky | Zendy

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Apoptosis of Growth Plate Chondrocytes Occurs through a Mitochondrial Pathway

Author(s) -

Cristina C. Teixeira,

Aida P. Padron Costas,

Yeleemelivsky

Publication year - 2007

Publication title -

the angle orthodontist

Language(s) - English

Resource type - Journals

eISSN - 1945-7103

pISSN - 0003-3219

DOI - 10.2319/062805-210r.1

Subject(s) - apoptosis , chondrocyte , microbiology and biotechnology , cytochrome c , mitochondrion , fragmentation (computing) , cytoplasm , dna fragmentation , mitochondrial apoptosis induced channel , mitochondrial dna , chemistry , biology , programmed cell death , biochemistry , in vitro , gene , ecology

Objective: To determine the role of mitochondria in chondrocyte apoptosis induced by inorganic phosphate (Pi). Materials and Methods: Chondrocytes isolated from the growth plates of chick embryo tibia were treated with Pi in serum-free media; chondrocyte viability, mitochondrial membrane potential, cytochrome c release from mitochondria, caspase 3 activity, endonuclease activity, and DNA fragmentation were investigated. Results: Exposure to Pi for 24 hours induced apoptosis in growth plate chondrocytes through a pathway that involved loss of mitochondrial function, release of cytochrome c into the cytoplasm, increases in caspase 3 and endonuclease activities, and fragmentation of DNA. Conclusions: This study suggests that mitochondria are important players in Pi-induced apoptosis.

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