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PCR amplification of Michele Tenore's historical specimens and facility to utilize an alternative approach to resolve taxonomic problems
Author(s) -
De Castro Olga,
Menale Bruno
Publication year - 2004
Publication title -
taxon
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 81
eISSN - 1996-8175
pISSN - 0040-0262
DOI - 10.2307/4135500
Subject(s) - herbarium , pinus brutia , chloroplast dna , biology , polymerase chain reaction , identification (biology) , botany , geography , pinus <genus> , genetics , phylogenetics , gene
A polymorphic noncoding region of chloroplast DNA (cpDNA) was amplified by the polymerase chain reaction (PCR) from herbarium specimens of Pinus brutia and P. halepensis . The samples belong to the historical collection of Michele Tenore. Several discordances and inconsistencies in taxonomic attribution are present for historical specimens of P. brutia . Moreover, there are inaccuracies as to their geographical distribution. In this work, we demonstrate the potential use of molecular methods of amplifying DNA from historical samples to resolve the identification of herbarium specimens.