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Organogenesis from internode‐derived nodules of Humulus lupulus var. Nugget (Cannabinaceae): histological studies and changes in the starch content
Author(s) -
Fortes Ana M.,
Pais Maria S.
Publication year - 2000
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.2307/2656996
Subject(s) - biology , primordium , shoot , explant culture , organogenesis , botany , callus , starch , tissue culture , biochemistry , gene , in vitro
The sequence of histological and histochemical events occurring during organogenesis from Humulus lupulus var. Nugget internode‐derived nodules was studied. Sections were made and studies were carried out from the start of culture treatment until the development of shoot buds. Cell division was observed in both cambial and cortical regions during the first week of culture establishment. Cell division in cortical cells led to the formation of an incipient callus tissue. From the calluses prenodular structures of cambial origin appeared and gave rise to nodules from which shoot buds formed. Nodules kept separating into “daughter nodules” from which arose an increasing number of shoot buds. Iodide staining showed a strong starch accumulation in callus tissue and in prenodular structures. During shoot‐bud primordia formation starch content decreased in nodules. Some starch was also noted in control explants (cultured on basal medium), however at a lower level than that observed in explants cultured on media with growth regulators. Shoot‐bud regeneration was not observed in control explants.