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Experimental design in intraspecific organelle DNA sequence studies I: haplotype detection methods
Author(s) -
Holt Sierra Stoneberg D.,
Bureš Petr
Publication year - 2007
Publication title -
taxon
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 81
eISSN - 1996-8175
pISSN - 0040-0262
DOI - 10.2307/25065744
Phylogenetic studies of organelle DNA sequences at low taxonomic levels present a researcher with specific experimental design challenges. Such studies require sampling of large numbers of individuals, many of which share identical haplotypes. The process is often limited by time and money required for DNA sequencing. A variety of mutation detection methods has been developed to circumvent this limitation. This paper briefly reviews the available methods and presents some of their advantages and disadvantages. The applicability of these methods to nuclear DNA studies is discussed.