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Successful extraction of DNA from 100‐year‐old herbarium specimens of the liverwort Bazzania trilobata
Author(s) -
Jankowiak Kamila,
Buczkowska Katarzyna,
Szweykowska-Kulinska Zofia
Publication year - 2005
Publication title -
taxon
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 81
eISSN - 1996-8175
pISSN - 0040-0262
DOI - 10.2307/25065361
Subject(s) - herbarium , dna extraction , dna , isolation (microbiology) , biology , botany , genetics , polymerase chain reaction , microbiology and biotechnology , gene
Using a modified DNeasy™ Plant Mini Kit method to extract DNA, we were able to isolate DNA from 100year‐old liverwort herbarium specimens. We sucessfully isolated DNA from 18 specimens of the liverwort Bazzania trilobata collected during the 20 th century. Our observations suggest that, at least over 100 years of material storage, the most crucial factor in successful DNA isolation is method of drying, not age of sample.