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Intraspecific variation and phylogeographic patterns of Fagus crenata (Fagaceae) mitochondrial DNA
Author(s) -
Tomaru Nobuhiro,
Takahashi Mizuki,
Tsumura Yoshihiko,
Takahashi Makoto,
Ohba Kihachiro
Publication year - 1998
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.2307/2446531
Subject(s) - biology , gene flow , intraspecific competition , biological dispersal , mitochondrial dna , genetic variation , population , restriction fragment length polymorphism , phylogeography , population genetics , haplotype , genetic diversity , evolutionary biology , genetics , zoology , gene , allele , phylogenetics , polymerase chain reaction , demography , sociology
Mitochondrial (mt) DNA variation in Japanese beech, Fagus crenata (Fagaceae), was studied in 17 populations distributed throughout the species’ range. Total genomic DNA of samples from single trees representing each of 12 populations were digested with 18 restriction enzymes and hybridized with three probes containing cox I, cox III, and atp A gene sequences. Thirty‐four of the 54 enzyme/probe combinations showed polymorphisms and all the individuals were subsequently analyzed with six combinations of three probes and two enzymes. Restriction fragment length polymorphisms were evident around all three genes, allowing the identification of eight distinct haplotypes. Haplotype diversity within the populations was found to be very low ( H S = 0.031), but population differentiation to be much higher ( G ST = 0.963). The mtDNA variation was strikingly different from allozyme variation ( H S = 0.209; G ST = 0.039). Gene flow for maternally inherited mtDNA should be restricted to seed dispersal while nuclear gene flow occurs by both seed and pollen dispersal. Therefore, the difference in the variation between mtDNA and allozymes may be largely a result of the much higher rate of gene flow associated with pollen dispersal than with seed dispersal. The mtDNA variation displayed strong geographic structure, which may reflect the species’ distribution in the last glacial maximum and subsequent colonization, and probably also reflects intraspecific phylogeography of the species.