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Using restriction‐site variation of PCR‐amplified cpDNA genes for phylogenetic analysis of Tribe Cheloneae (Scrophulariaceae)
Author(s) -
Wolfe Andrea D.,
Elisens Wayne J.,
Watson Linda E.,
dePamphilis Claude W.
Publication year - 1997
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.2307/2446031
Subject(s) - biology , polyphyly , monophyly , phylogenetic tree , scrophulariaceae , chloroplast dna , clade , phylogenetics , tribe , restriction site , evolutionary biology , botany , genetics , restriction enzyme , gene , sociology , anthropology
Data from restriction‐site variation of three PCR‐amplified chloroplast genic regions ( trn K, rps 2, and rbc L) were used to assess the utility of PCR‐based methodology for phylogenetic reconstruction. Seventeen genera from tribe Cheloneae s.l. (Scrophulariaceae), and one genus each from Solanaceae, Acanthaceae, and Bignoniaceae, representing 32 taxa, were sampled. Phylogenetic reconstruction, based on a combined data set of 138 variable restriction sites, revealed a monophyletic clade of North American Cheloneae, which were not inconsistent with a polyphyletic Scrophulariaceae. Separate analyses of individual genic regions were unable to completely resolve the phylogeny, but were adequate for resolving relationships of major clades among the taxa sampled. We suggest that analysis of PCR‐product restriction‐site variation is useful for phylogenetic reconstruction above the species level.