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Medium Perfusion in Cultures of Small Aquatic Animals
Author(s) -
Salt George W.
Publication year - 1965
Publication title -
ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.144
H-Index - 294
eISSN - 1939-9170
pISSN - 0012-9658
DOI - 10.2307/1935278
Subject(s) - volume (thermodynamics) , population , biology , filtration (mathematics) , ecology , capillary action , environmental science , chemistry , pulp and paper industry , materials science , thermodynamics , mathematics , statistics , physics , demography , sociology , engineering , composite material
For experiments on animal populations which run for extended periods of time, a method of removal of medium is needed in order to avoid accumulation of metabolites and inhibition of population processes. This can be done for cultures of small aquatic organisms, such as protozoa, by using a filter paper wick which removes fluid by capillary action. The animals are not removed. A like volume of fresh fluid is added after each removal to maintain a constant volume. To prevent drying of the wick, the culture dish and wick are kept in a saturated atmosphere inside a glass cabinet or box.

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