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An improved method of DNA isolation from plants collected in the field and conserved in saturated NaCl/CTAB solution
Author(s) -
Štorchová Helena,
Hrdličková Radmila,
Chrtek Jindřich,
Tetera Martin,
Fitze Dorothee,
Fehrer Judith
Publication year - 2000
Publication title -
taxon
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 81
eISSN - 1996-8175
pISSN - 0040-0262
DOI - 10.2307/1223934
Subject(s) - rapd , dna extraction , dna , genomic dna , biology , isolation (microbiology) , botany , polymerase chain reaction , chromatography , microbiology and biotechnology , chemistry , biochemistry , gene , population , demography , sociology , genetic diversity
Summary Štorchová, H., Hrdličková, R., Chrtek, J., Jr., Tetera, M., Fitze, D. & Fehrer, J.: An improved method of DNA isolation from plants collected in the field and conserved in saturated NaCl/CTAB solution. – Taxon 49: 79‐84. 2000. – ISSN 0040‐0262. A simple method for isolation of genomic DNA from wild plants sampled in remote field areas is presented. The protocol combines NaCl/CTAB leaf preservation with sorbitol extraction of secondary compounds which often contain inhibitors of Taq DNA polymerase activity. The obtained DNA is suitable for random amplified polymorphic DNA (RAPD) analysis of plant populations as well as for specific amplification of chloroplast DNA sequences. The NaCl/CTAB leaf preservation is a powerful alternative to silica gel drying‐based preservation.