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Taxonomic study of Taiwan Astragalus based on genetic variation
Author(s) -
Luo MiawChyi,
Hwu KaeKang,
Huang TsengChieng
Publication year - 2000
Publication title -
taxon
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 81
eISSN - 1996-8175
pISSN - 0040-0262
DOI - 10.2307/1223930
Subject(s) - rapd , upgma , biology , jaccard index , genetic relationship , taxon , astragalus , genetic variation , genetic distance , genetic similarity , genetic diversity , botany , genetics , population , statistics , demography , medicine , pathology , cluster analysis , sociology , gene , mathematics , alternative medicine , traditional chinese medicine
Summary Luo, Miaw‐Chyi, Hwu, Kae‐Kang & Huang, Tseng‐Chieng: Taxonomic study of Taiwan Astragalus based on genetic variation. – Taxon 49: 35‐46. 2000. – ISSN 0040‐0262. Three species of Astragalus, A. sinicus L., A. nokoensis Sasaki, and A. nankotaizanensis Sasaki, occur in Taiwan, and the latter two are endemic. Randomly amplified polymorphic DNA (RAPD) analysis was used to determine genetic variation of the three species. Bands in RAPD profiles were treated as independent loci. From studies of 207 individuals in eight populations of Astragalus , a total of 131 RAPD bands was generated using eleven 10‐mer primers. Genetic similarity among 207 individuals in the eight populations was calculated using Jaccard's coefficient. The results of the UPGMA cluster analysis were in broad agreement with previous classifications of these three species, which clearly discriminated between Astragalus sinicus, A. nankotaizanensis , and A. nokoensis. Based on RAPD data, A. nankotaizanensis and A. nokoensis were slightly closer than A. nokoensis and A. sinicus. A positive relationship between genetic distance and geographical distance for populations of A. nokoensis was also seen. The results of this study do not support classification of these three species into one species as A. sinicus s.l.