Open AccessIdentification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L.
Author(s) -
Maja Ignjatov,
A Slobodan Vlajic,
Nenad Milošević,
Toni Nikolić,
D Gordana Tamindzic,
M Jelica Gvozdanovic-Varga,
Slobodan Ivanović
Publication year - 2019
Publication title -
zbornik matice srpske za prirodne nauke
Language(s) - English
Resource type - Journals
eISSN - 2406-0828
pISSN - 0352-4906
DOI - 10.2298/zmspn1937049i
Subject(s) - fusarium proliferatum , biology , mycelium , gibberella , fusarium , allium , phylogenetic tree , botany , polymerase chain reaction , pathogenicity , gibberella fujikuroi , fungus , microbiology and biotechnology , gene , genetics
Symptoms of cloves rot of A. ampeloprasum were noticed during 2018 in storage conditions. 16 isolates were obtained (AMP1-AMP16) and according to morphological and cultural characteristics they belong to F. proliferatum (sex. stage Gibberella intermedia), species of Gibberella fujikuroi complex. To confirm morphological identifica?tion, total genomic DNA was extracted from mycelium of the 16 isolates by amplification of TEF-1? gene, using polymerase chain reaction (PCR) that was performed with the primer pair EF1 and EF2. Results presented in this article clearly indicated that the new host of Fusarium proliferatum as the causal agent of cloves rot is ?elephant garlic? Allium ampeloprasum. Pathogenicity test was confirmed on Allium ampeloprasum cloves. Pathogenicity assays revealed that all isolates caused symptoms on tested Allium spp., like naturally infected cloves.