Open AccessAnalysis of aflatoxins B1 and G1 in maize by quechers
Author(s) -
Vojislava Bursić,
Gorica Vuković,
Igor Jajić,
Sanja Lazić,
Magdalena Cara,
Radmilo Čolović,
Đuro Vukmirović
Publication year - 2013
Publication title -
zbornik matice srpske za prirodne nauke
Language(s) - English
Resource type - Journals
eISSN - 2406-0828
pISSN - 0352-4906
DOI - 10.2298/zmspn1324051b
Subject(s) - quechers , aflatoxin , chromatography , mycotoxin , derivatization , extraction (chemistry) , high performance liquid chromatography , reproducibility , detection limit , chemistry , food science , biology , pesticide , pesticide residue , agronomy
A reliable and easy method has been developed for the determination of aflatoxins B1 and G2 in maize samples. High performance liquid chromatography coupled with FLD (HPLC-FLD) with photochemical derivatization was used. Mycotoxins were extracted from maize using a QuEChERS-based extraction procedure. The optimized analytical conditions were evaluated in terms of recoveries, reproducibility, LOD, LOQ and linearity for aflatoxin B1 and aflatoxin G1 in maize. Extraction, chromatographic and detection conditions were optimized in order to increase sample sensitivity. The linearity was analyzed in the range of 0.4-20 μg/kg and the correlation coefficients (R2) were higher than 0.99 for aflatoxins B1 and G1. Blank samples were spiked at 1.0, 2.0 and 4.0 μg/kg, and the average recovery for aflatoxin G1 was 96.96±1.72% and for aflatoxin B1 it was 86.80±1.24%. RSDs were lower than 25% for both mycotoxins. LOD for both aflatoxins was 0.5 μg/kg and LOQ was 1.0 μg/kg, respectively