Open AccessLigninolytic enzyme production in Pleurotus eryngii depending on the medium composition and cultivation conditions
Author(s) -
Mirjana Stajić,
N Sonja Duletic-Lausevic,
Jelena Vukojevic
Publication year - 2005
Publication title -
zbornik matice srpske za prirodne nauke
Language(s) - English
Resource type - Journals
eISSN - 2406-0828
pISSN - 0352-4906
DOI - 10.2298/zmspn0508269s
Subject(s) - pleurotus eryngii , chemistry , food science , laccase , fermentation , phenol , nitrogen , composition (language) , solid state fermentation , dry weight , pleurotus , botany , enzyme , biochemistry , mushroom , biology , organic chemistry , linguistics , philosophy
Pleurotus eryngii produced laccase (Lac) both under conditions of submerged fermentation (SF) and solid-state fermentation (SSF) using all of the investigated carbon and nitrogen sources, while significant peroxidases production occurred only under SSF conditions. The highest levels of Lac activity were found under SF conditions of dry ground mandarine peels (999.5 U/l). After purification of extracellular crude enzyme mixture of P. eryngii which was grown under SF conditions with dry ground mandarine peels it was revealed two peaks of Lac activity and one peak of activity against phenol red in absence of external Mn2+ which was very low (1.4 U/l). Results obtained by purification also showed that the levels of phenol red oxidation in absence of external Mn2+ were higher than phenol red oxidation levels in presence of external Mn2+. In the medium with the best carbon source for Lac production (dry ground mandarine peels), (NH4)2SO4, with a nitrogen concentration of 20 mM, was the most optimum nitrogen source among 8 investigated sources