Open AccessAltered high-density lipoprotein particle structure and antioxidant capacity in preeclampsia
Author(s) -
Gorica Banjac,
Daniela Ardalić,
Petar Cabunac,
Jasmina Ivanišević,
Jelena Vekić,
Željko Miković,
Aleksandra Stefanović
Publication year - 2023
Publication title -
vojnosanitetski pregled
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.123
H-Index - 19
eISSN - 2406-0720
pISSN - 0042-8450
DOI - 10.2298/vsp210413010b
Subject(s) - paraoxonase , preeclampsia , pon1 , medicine , pregnancy , lipoprotein , high density lipoprotein , blood pressure , endocrinology , obstetrics , chemistry , cholesterol , biology , biochemistry , oxidative stress , gene , genotype , genetics
Background/Aim. The aim of this study was to investigate the structural and functional modifications of HDL particles longitudinally through a high-risk pregnancy for preeclampsia development, in women who remained under the risk until the delivery and those who developed preeclampsia. Methods. The study group included 71 pregnant women who remained under risk of developing preeclampsia until delivery and 20 pregnant women who developed preeclampsia. The blood was sampled toward the end of each trimester and before the delivery. The distribution of HDL particles was determined by the vertical 3-31% polyacrylamide gradient gel electrophoresis method. The antioxidative capacity of HDL particles was measured by the action of HDL-associated antioxidative enzyme, paraoxonase 1 (PON1). PON1 activity was determined by the kinetic spectrophotometric method in serum. Results. The results have shown that the proportions of HDL 2b particles significantly increased in the 2nd trimester (P?0.05) and remained higher until the end of pregnancy in the high-risk group. PON1 activity was significantly higher in the 3rd trimester (P<0.05) of the high-risk pregnancy group. In the preeclampsia group, we found that the proportions of HDL 3a particles significantly decreased in the 2nd trimester (P<0.05) and stayed lower until the end of pregnancy. PON1 activity was not changed during the pregnancy with preeclampsia. Conclusion. Dyslipidemia in pregnancy could be associated with different modifications of HDL particles. Adaptive pregnancy mechanisms expressed as a functional modification of HDL particles in pregnant women with high risk for preeclampsia seem to lack.