
Investigation of aflatoxin M1 degradation in milk
Author(s) -
Ahmed Smajlović,
Mehmed Muminović,
Indira Mujezinović,
Vitormir Cupic
Publication year - 2012
Publication title -
veterinarski glasnik
Language(s) - English
Resource type - Journals
eISSN - 2406-0771
pISSN - 0350-2457
DOI - 10.2298/vetgl1206387s
Subject(s) - aflatoxin , metabolite , contamination , food science , chemistry , absorbance , microwave oven , toxin , mycotoxin , microwave , chromatography , biology , biochemistry , ecology , physics , quantum mechanics
Aflatoxin M1 is a highly toxic 4-hydroxylated metabolite of aflatoxins B1 and B2. It is one of the most potent hepatocarcinogens, mutagens, teratogens and immunosuppressors. Feed is often contaminated with aflatoxigenic moulds and aflatoxins with a high possibility of contaminating milk and dairy products with aflatoxin M1. Samples of artificially contaminated milk were exposed to the effects of physical conditions (temperature of -18oC and for microwaves in a microwave oven), time (during the period from 1 to 12 months) and a combination of the above mentioned conditions. Following this, levels of aflatoxin M1 degradation were established by using the ELISA method. An insignificant decrease in concentration of toxin was observed which indicates that a temperature of -18°C does not significantly influence the concentration of aflatoxin M1 in the artificially contaminated milk. At the same time, treatment of milk with microwaves in a microwave oven showed an insignificant influence on the percentage of aflatoxin M1 absorbance