Open AccessInvestigations of hemadsorption characteristics of glycoprotein HN and F antigens of Newcastle Disease virus envelope in vitro
Author(s) -
Jakov Nišavić,
Nataša Milić
Publication year - 2006
Publication title -
veterinarski glasnik
Language(s) - English
Resource type - Journals
eISSN - 2406-0771
pISSN - 0350-2457
DOI - 10.2298/vetgl0606299n
Subject(s) - newcastle disease , serial dilution , virus , antigen , virology , trypsin , inoculation , vero cell , biology , glycoprotein , in vitro , microbiology and biotechnology , medicine , immunology , biochemistry , enzyme , alternative medicine , pathology
The objective of these investigations was to examine the hemadsorption characteristics of glycoprotein HN and F antigens of the Newcastle Disease virus envelope. Six hours following inoculation of the cell line with the Newcastle Disease Vero virus, previously activated with 0.025 g/dl trypsin-versene, the appearance of hemadsorption was established in a smaller intensity in antigens diluted in ratios from 1:8 to 1:16. Hemadsorption was also determined in the mentioned cell lines 12 hours following inoculation in viruses diluted 1:64, while hemadsorption was present in virus dilutions of 1:256 after 24 hours. Inhibition of hemadsorption in inoculated cells with immune serum dilutions of 1:64 was established 24 hours following inoculation of the cell line with Vero samples of the Newcastle Disease virus, activated with trypsin-versene and the addition of specific immune serum