Open AccessLipid and glycogen contents in liver of high-yield dairy cows in peripartal period
Author(s) -
Radojica Đoković,
Horea Šamanc
Publication year - 2004
Publication title -
veterinarski glasnik
Language(s) - English
Resource type - Journals
eISSN - 2406-0771
pISSN - 0350-2457
DOI - 10.2298/vetgl0402077d
Subject(s) - glycogen , hepatocyte , infiltration (hvac) , pathological , histopathology , biopsy , cellular infiltration , liver biopsy , pathology , lactation , medicine , necrosis , biology , endocrinology , pregnancy , biochemistry , in vitro , physics , genetics , inflammation , thermodynamics
Liver tissue samples were taken by biopsy from Holstein cows in advanced stages of gravidity and in early lactation for pathological-histological examinations. Lipid content in hepatocytes was determined using the stereometric method by calculating volume density, and of glycogen using semi-quantitative microscopic examination of sections stained according to the method of Best. Pathological-histological examinations of liver tissue samples in healthy animals, gravid or peripartal cows did not reveal lipid infiltration or cell degeneration, and hepatocytes were completely or partly filled with glycogen. In ketotic cows, pathological-histological examinations of liver tissue samples showed lipid infiltration and hepatocyte degeneration of different intensity. In only one ketotic cow, we determined a slight degree of lipid infiltration, there was a medium degree of lipid infiltration and degeneration in six cows, and three cows were found to have a grave form of fatty liver. The quantity of glycogen in hepatocytes is in negative correlation with the degree of lipid infiltration and degeneration. In severe cases of fatty liver, glycogen is completely absent from hepatocyte cytoplasm