Open AccessGenotoxicity test methods - a tool for DNA and chromosome damage biomonitoring
Author(s) -
Nevenka Veličkova,
M Milev
Publication year - 2020
Publication title -
srpski arhiv za celokupno lekarstvo
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.135
H-Index - 17
eISSN - 2406-0895
pISSN - 0370-8179
DOI - 10.2298/sarh200114060v
Subject(s) - genotoxicity , micronucleus test , comet assay , biomonitoring , sister chromatid exchange , chromosome aberration , medicine , micronucleus , sister chromatids , toxicology , dna damage , genetics , computational biology , chromosome , biology , dna , toxicity , ecology , gene
Nowadays, life is highly influenced by intense growth of various industries, high levels of pollution, and other environmental factors with harmful effects on human health. Therefore, cytogenetic monitoring is essential for detection of changes in the structure of chromosomes, which occur because of the effects of various genotoxic agents. In this review, we shall apprize the theoretical and experimental aspects of several tests for assessment of genotoxicity in humans such as Micronucleus assay, Comet assay, Chromosomal aberrations assessment and Sister chromatid exchanges analysis. These methods are accepted by the World Health Organization as standard tests for genotoxicological screening in humans. The methods are sensitive and confirm the cellular genotoxic effects of various genotoxicants.