Open AccessModification of peritoneal dialysis model on rabbits: A pilot study
Author(s) -
Snežana Žunić-Božinovski,
Biljana Stojimirović,
Ž. Laušević,
S Krstić,
Jasna Trbojević-Stanković,
Nenad Jovanović
Publication year - 2008
Publication title -
srpski arhiv za celokupno lekarstvo
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.135
H-Index - 17
eISSN - 2406-0895
pISSN - 0370-8179
DOI - 10.2298/sarh08s1038z
Subject(s) - medicine , peritoneal dialysis , peritoneal cavity , dialysis , body weight , surgery , catheter , exit site , abdomen , urology
. Long-term peritoneal dialysis, a well-established method of depuration in end-stage renal disease patients, is assosiated with morphological and functional alterations of the peritoneal membrane due to the use of bioincompatible dialysis solutions. Studying effects of dialysate on the peritoneal tissue in humans is still a challenge due to ethical and technical limitations. There has been a variety of peritoneal dialysis experimental models but without consensus on the ideal model so far. OBJECTIVE. We aimed to develop a new, modified experimental rabbit model of peritoneal dialysis which would be practical, easy to conduct, relatively inexpensive and convenient to study long-term effects of dialysis solution on the peritoneal membrane. METHOD. This pilot study was performed on five healthy Chinchilla rabbits of both sexes. After i.v. Thiopental injection BP 1G, 0.5 ml/kg body mass, a catheter, especially made from Tro-soluset (Troge Medical GmbH, Hamburg, Germany) infusion system, was surgically tunneled from the animals? neck to the abdomen and inserted to the bottom of the peritoneal cavity. After one week recovery period, peritoneal dialysate instillations were performed for four weeks. During the whole five week experimental period a follow-up diary was kept. RESULTS. All procedures were well tolerated by the animals. The rabbits gained body weight, had normal body temperature and no complications were noted. CONCLUSION. The presented modified peritoneal dialysis model is practical, reproducible, does not require sophisticated technology and is well tolerated by the animals. That is why it is convenient for studying long-term effects of dialysate on the rabbit?s peritoneal membrane.