Open AccessApplication of different combinations of lactic acid, phototrophic bacteria and yeast mixtures in control of seed and seedlings pathogens of tomato and pepper
Author(s) -
Danijela Ristić,
Ivan Vučurović,
Goran Aleksić,
Bogdan Nikolić,
Sanja Djurovic,
Mira Starović
Publication year - 2021
Publication title -
pesticidi i fitomedicina
Language(s) - English
Resource type - Journals
eISSN - 2406-1026
pISSN - 1820-3949
DOI - 10.2298/pif2102073r
Subject(s) - biology , alternaria alternata , botrytis cinerea , fusarium oxysporum , pythium aphanidermatum , lactobacillus plantarum , horticulture , botany , microbiology and biotechnology , bacteria , lactic acid , biological pest control , genetics
Application of three combinations of lactic acid bacteria (Lactobacillus plantarum, Lactobacillus rhamnosus), phototrophic bacteria (Rhodopseudomonas palustris) and yeast (Saccharomyces cerevisiae) with sugar cane molasses, marked as: EM1, EM5 and EM AGRO, against the phytopathogenic fungi of tomato and pepper: Fusarium oxysporum, Alternaria alternata, Botrytis cinerea, Colletotrichum sp., Verticilium dahliae and Pythium aphanidermatum was evaluated in vitro and in vivo. A combination of bacteria and yeast named EM5 showed the highest mycelium growth inhibition against B. cinerea (38.4%) in a double agar diffusion test. In a microdilution test, the combination EM1 showed the highest inhibitory effect on B. cinerea (MIC 1x10-3 ?l/ml), while EM5 showed a similar inhibitory effect towards F. oxysporum, A. alternata and Colletotrichum sp. (MIC 10 ?l/ml). The use of EM1 (in concentrations 10 and 100 ?l/ml) and EM AGRO (10 ?l/ml) is recommended for tomato seedling protection. ??1 (100 ?l/ml), ??5 and ?? AGRO (10 ?l/ml) are recommended for pepper seedling protection.