Open AccessElaboration of methods for detection of Xanthomonas axonopodis pv. phaseoli on bean seeds
Author(s) -
Jelica Balaž,
Tatjana Popovič,
Mirjana Vasić,
Zorica Nikolić
Publication year - 2008
Publication title -
pesticidi i fitomedicina
Language(s) - English
Resource type - Journals
eISSN - 2406-1026
pISSN - 1820-3949
DOI - 10.2298/pif0802089b
Subject(s) - biology , agar , isolation (microbiology) , xanthomonas campestris , inoculation , pathogenicity , bacteria , xanthomonas , agar plate , gram , microbiology and biotechnology , horticulture , botany , genetics
Xanthomonas axonopodis pv. phaseoli detection on artificially inoculated bean seeds was investigated. The method of the International Seed Federation - ISF (2006) was used. It included bacteria extraction from seeds and isolation on semiselective media with pathogenicity test of the investigated isolates. ELISA and PCR were used for verification of results. The results showed that the semiselective media MT (Milk Tween Agar) and XCP1 (Xanthomonas campestris pv. phaseoli Agar) were very suitable for isolation of X. a. pv. phaseoli. Pathogenicity was confirmed on young bean plants. ELISA test and PCR confirmed that all investigated isolates and reisolates belong to the bacterium X. a. pv. phaseoli.