Open AccessElaboration of methods for detection of Pseudomonas savastanoi pv. phaseolicola on bean seeds
Author(s) -
Jelica Balaž,
Tatjana Popović,
Mirjana Vasić,
Zorica Nikolić
Publication year - 2008
Publication title -
pesticidi i fitomedicina
Language(s) - English
Resource type - Journals
eISSN - 2406-1026
pISSN - 1820-3949
DOI - 10.2298/pif0802081b
Subject(s) - biology , agar , isolation (microbiology) , bacteria , pseudomonas , pathogenicity , inoculation , nutrient agar , microbiology and biotechnology , agar plate , pseudomonadaceae , pseudomonas syringae , pathogen , horticulture , genetics
Pseudomonas savastanoi pv. phaseolicola detection on artificially inoculated bean seeds was investigated. The method of the International Seed Federation - ISF (2006) was used. It includes bacteria extraction from seeds, isolation on semiselective media and checking the pathogenicity of investigated isolates. For verification of results, quick new methods of investigation were used (ELISA test and PCR). The results show that semiselective media MT (Milk Tween Agar) and MSP (Modified Sucrose Peptone Agar) can be appropriate for isolation of this bacterium. Pathogenicity of the investigated isolates was confirmed on cotyledon leaves of bean. ELISA test and PCR confirmed that all investigated isolates and reisolates belong to the bacterium P. s. pv. phaseolicola.