Open AccessIn vitro production of human dermal equivalent
Author(s) -
Zoran Milosavljević,
Biljana Ljujić
Publication year - 2010
Publication title -
medicinski pregled
Language(s) - English
Resource type - Journals
eISSN - 1820-7383
pISSN - 0025-8105
DOI - 10.2298/mpns1008459m
Subject(s) - foreskin , connective tissue , in vitro , fibroblast , in vivo , type i collagen , explant culture , human skin , medicine , tissue culture , extracellular matrix , dissociation (chemistry) , cell culture , microbiology and biotechnology , andrology , pathology , chemistry , biochemistry , biology , genetics
Human dermal tissue is composed of loose and dense connective tissue. Main cell populations are fibroblasts and the dominant fibers are built from collagen type I. The aim of our study was to determine the precise method and time frame for the in vitro production of human dermal equivalent and to investigate the effects of ratio of structural elements and vitamin C on characteristics of the engineered tissue.